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KMID : 0903519760190030139
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1976 Volume.19 No. 3 p.139 ~ p.144
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Studies on the Production of Fermented Feeds from Agricultural Waste Products(Part Ⅳ)
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Abstract
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In order to investigate the properties of enzymes from two strains of mold, reported in the previous paper, (1) studies have been made concerning the characteristics of cellulose cf Aspergillus niger-SM6 and Trichoderma viride-SM10, and summarized as follows.
1. In the semi-purification the recovery of β-glucosidase was the highest when 80-90% ethanol was used and 0.8 saturation of (NH₄)₂SO₄.
2. The characteristics of the semi-purified enzyme were as follows.
Mspergillus niger-SM6 Trichoderma viride-SV110
Optimum pH 3.5 4.0
pH stability 3.0-6.0 3.0-6.0
Optimum temperature 60℃ 60℃
Heat stability below 60℃ below 50℃
Optimum reaction time 30 min. 60 min.
Optimum CMC concentration 3% 3%
3. The Km values of CMCase were 0.8% and 1.01 for Aspergillus niger-SM6 and Trichoderma viride-SM10, respectively.
4. In the strain of Aspergillus niger-SM6, there were high activity of xylanase and pectinase.
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